Anti-C5a Antibody | Boster Immunoleader PB9187 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-C5a Antibody

catalog :

PB9187

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-C5a Antibody

Catalog Number :

PB9187

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Rat

Notes :

WB: The detection limit for C5a is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. C5a plays a key role in increasing migration and adherence of neutrophils and monocytes to vessel walls. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Reference :

1. Delgado-Cervino, E., Fontan, G., Lopez-Trascara, M. C5 complement deficiency in a Spanish family: molecular characterization of the double mutation responsible for the defect. Molec. Immun. 42: 105-111, 2005. 2. Pfarr, N., Prawitt, D., Kirschfink, M., Schroff, C., Knuf, M., Habermehl, P., Mannhardt, W., Zepp, F., Fairbrother, W. G., Loos, M., Burge, C. B., Pohlenz, J. Linking C5 deficiency to an exonic splicer enhancer mutation. J. Immun. 174: 4172-4177, 2005. Note: Erratum: J. Immun. 182: 5152 only, 2009.

Gene Name :

Protein Name :

Complement C5

Gene Full Name :

complement component 5

Synonyms :

Anaphylatoxin C5a analog antibody Anaphylatoxin C5a antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody C5 antibody C5a antibody CO5_HUMAN antibody Complement C5 alpha'' chain antibody Complement C5 antibody Complement component C5 antibody Complement component C5a antibody CPAMD 4 antibody CPAMD4 antibody

Uniprot ID :

P08650

more info or order :

Boster Immunoleader product webpage