Anti-CD82 Antibody | Boster Immunoleader PB9170 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD82 Antibody

catalog :

PB9170

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CD82 Antibody

Catalog Number :

PB9170

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for CD82 antigen(CD82) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for CD82 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD82(Cluster of Differentiation 82), also named KAI1, is a protein that in humans encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma(ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.

Reference :

1. Dong, J.-T.; Lamb, P. W.; Rinker-Schaeffer, C. W.; Vukanovic, J.; Ichikawa, T.; Isaacs, J. T.; Barrett, J. C. : KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886, 1995. 2. Mashimo, T.; Watabe, M.; Hirota, S.; Hosobe, S.; Miura, K.; Tegtmeyer, P. J.; Rinker-Shaeffer, C. W.; Watabe, K. : The expression of the KAI1 gene, a tumor metastasis suppressor, is directly activated by p53. Proc. Nat. Acad. Sci. 95: 11307-11311, 1998. 3. Miyazaki, T.; Kato, H.; Shitara, Y.; Yoshikawa, M.; Tajima, K.; Masuda, N.; Shouji, H.; Tsukada, K.; Nakajima, T.; Kuwano, H. : Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer 89: 955-962, 2000. 4. Jee, B. K.; Park, K. M.; Surendran, S.; Lee, W. K.; Han, C. W.; Kim, Y. S.; Lim, Y. : KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line. Biochem. Biophys. Res. Commun. 342: 655-661, 2006.

Gene Name :

CD82

Protein Name :

CD82 antigen

Gene Full Name :

CD82 molecule

Synonyms :

4F9 antibody Antigen detected by monoclonal and antibody IA4 antibody C33 antibody C33 antigen antibody CD 82 antibody CD82 antibody Cd82 antibody CD82 antigen antibody CD82 molecule antibody CD82_HUMAN antibody GR 15 antibody GR15 antibody IA 4 antibody IA4 antibody Inducible membrane protein antibody Inducible membrane protein R2 antibody KAI 1 antibody KAI1 antibody Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody Kangai 1 antibody Kangai1 antibody Leukocyte surface antigen R2 antibody Metastasis suppressor Kangai 1 antibody Metastasis suppressor Kangai-1 antibody Metastasis suppressor Kangai1 antibody Prostate cancer antimetastasis gene KAI1 antibody R2 antibody R2 leukocyte antigen antibody SAR 2 antibody SAR2 antibody ST 6 antibody ST6 antibody Suppression of tumorigenicity 6 antibody Suppression of tumorigenicity 6 prostate antibody Suppressor of tumorigenicity 6 antibody Suppressor of tumorigenicity 6 protein antibody Tetraspanin 27 antibody Tetraspanin-27 antibody Tetraspanin27 antibody Tspan 27 antibody Tspan-27 antibody Tspan27 antibody

Uniprot ID :

P27701

more info or order :

Boster Immunoleader product webpage