Boster Immunoleader

antibody

Anti-B Raf Antibody

PB9164

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

Anti-B Raf Antibody

PB9164

Polyclonal

Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for B Raf is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

1. Sithanandam G, Kolch W, Duh FM, Rapp UR (December 1990). "Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies". Oncogene 5 (12): 1775–80. 2. Sithanandam G, Druck T, Cannizzaro LA, Leuzzi G, Huebner K, Rapp UR (April 1992). "B-raf and a B-raf pseudogene are located on 7q in man". Oncogene 7 (4): 795–9. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.

BRAF

Serine/threonine-protein kinase B-raf

B-Raf proto-oncogene, serine/threonine kinase

FLJ95109 antibody 94 kDa B raf protein antibody B raf 1 antibody B raf 1 antibody B Raf proto oncogene serine threonine protein kinase antibody B Raf proto oncogene serine threonine protein kinase antibody B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody BRAF 1 antibody BRaf antibody BRaf antibody BRAF_HUMAN antibody BRAF1 antibody BRAF1 antibody cRmil antibody MGC126806 antibody MGC138284 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral (v-raf) oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody Murine sarcoma viral v raf oncogene homolog B1 antibody NS7 antibody oncogene BRAF1 antibody p94 antibody p94 antibody Proto-oncogene B-Raf antibody Proto-oncogene c-Rmil antibody RAFB 1 antibody RAFB1 antibody RAFB1 antibody RMIL antibody Serine/threonine-protein kinase B-raf antibody v raf murine sarcoma viral oncogene homolog B antibody v raf murine sarcoma viral oncogene homolog B1 antibody v raf murine sarcoma viral oncogene homolog B1 antibody v-Raf murine sarcoma viral oncogene homolog B1 antibody

P15056