Boster Immunoleader

antibody

Anti-PROM1 Antibody

PB9156

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-PROM1 Antibody

PB9156

Polyclonal

Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for PROM1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human PROM1 recombinant protein (Position: P531-H865). Human PROM1 shares 61% amino acid (aa) sequence identity with mouse PROM1.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.

1. Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW (1997). "AC133, is a novel marker for human hematopoietic stem and progenitor cells". Blood 90 (12): 5002–5012. 2. Corbeil D, Fargeas CA, Huttner WB (2001). "Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein". Biochem Biophys Res Commun 285 (4): 939–44. 3. Shmelkov, S. V., Butler, J. M., Hooper, A. T., Hormigo, A., Kushner, J., Milde, T., St. Clair, R., Baljevic, M., White, I., Jin, D. K., Chadburn, A., Murphy, A. J., Valenzuela, D. M., Gale, N. W., Thurston, G., Yancopoulos, G. D., D'Angelica, M., Kemeny, N., Lyden, D., Rafii, S. CD133 expression is not restricted to stem cells, and both CD133+ and CD133- metastatic colon cancer cells initiate tumors. J. Clin. Invest. 118: 2111-2120, 2008.

PROM1

Prominin-1

prominin 1

AC133 Antigen AC133 CD133 antibody CORD12 hematopoietic stem cell antigen hProminin MCDR2 MSTP061 antibody PROM1 PROM 1 PROM-1 Prominin-1 Prominin 1 Prominin1 Prominin-like protein 1 PROML1 antibody RP41 STGD4

O43490