Anti-SLC9A1 Antibody | Boster Immunoleader PB9151 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SLC9A1 Antibody

catalog :

PB9151

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-SLC9A1 Antibody

Catalog Number :

PB9151

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 1(SLC9A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

WB: The detection limit for SLC9A1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Reference :

1. Fliegel L, Dyck JR, Wang H, Fong C, Haworth RS (August 1993). "Cloning and analysis of the human myocardial Na+/H+ exchanger". Mol. Cell. Biochem. 125 (2): 137–43. 2. Mattei MG, Sardet C, Franchi A, Pouysségur J (1988). "The human amiloride-sensitive Na+/H+ antiporter: localization to chromosome 1 by in situ hybridization". Cytogenet. Cell Genet. 48 (1): 6–8. 3. Denker, S. P., Huang, D. C., Orlowski, J., Furthmayr, H., Barber, D. L. Direct binding of the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Molec. Cell 6: 1425-1436, 2000. 4. Dudley, C. R. K., Giuffra, L. A., Tippett, P., Kidd, K. K., Reeders, S. T. The Na+/H+ antiporter: a 'melt' polymorphism allows regional mapping to the short arm of chromosome 1. Hum. Genet. 86: 79-83,1990.

Gene Name :

SLC9A1

Protein Name :

Sodium/hydrogen exchanger 1

Gene Full Name :

solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

Synonyms :

amiloride-sensitive antibody APNH antibody APNH1 antibody FLJ42224 antibody Na Li countertransporter antibody Na(+)/H(+) antiporter antibody Na(+)/H(+) exchanger 1 antibody Na+ H+ antiporter amiloride-sensitive antibody Na+ H+ antiporter antibody Na+ H+ exchanger 1 antibody NHE-1 antibody NHE1 antibody OTTHUMP00000004468 antibody SL9A1_HUMAN antibody SLC9A1 antibody Sodium hydrogen exchanger 1 antibody Sodium/hydrogen exchanger 1 antibody solute carrier family 9 antibody Solute carrier family 9 member 1 antibody Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody Solute carrier family 9 subfamily A member 1 antibody

Uniprot ID :

P19634

more info or order :

Boster Immunoleader product webpage