Boster Immunoleader

antibody

Anti-Bub1 Antibody

PB9135

100 ug/vial

240 USD

polyclonal

rabbit

human, rat

Anti-Bub1 Antibody

PB9135

Polyclonal

Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1(BUB1) detection. Tested with WB in Human;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

WB: The detection limit for Bub1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

1. "Entrez Gene: BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)" 2. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B (March 1998). "Mutations of mitotic checkpoint genes in human cancers". Nature 392 (6673): 300–3. 3. Roberts BT, Farr KA, Hoyt MA (December 1994). "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase". Mol. Cell. Biol. 14 (12): 8282–91.

BUB1

Mitotic checkpoint serine/threonine-protein kinase BUB1

BUB1 mitotic checkpoint serine/threonine kinase

Bub1 antibody BUB1 budding uninhibited by benzimidazoles 1 homolog antibody BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody BUB1 mitotic checkpoint serine/threonine kinase antibody BUB1, S. cerevisiae, homolog of antibody BUB1_HUMAN antibody BUB1A antibody BUB1L antibody Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody Budding uninhibited by benzimidazoles 1 homolog antibody Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody hBUB1 antibody Homolog of mitotic checkpoint gene BUB1 antibody Mitotic checkpoint gene BUB1 antibody Mitotic checkpoint serine/threonine protein kinase BUB1 antibody Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody Mitotic spindle checkpoint kinase antibody Putative serine/threonine protein kinase antibody

O43683