Anti-RIP Antibody | Boster Immunoleader PB9116 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-RIP Antibody

catalog :

PB9116

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-RIP Antibody

Catalog Number :

PB9116

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for RIP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human RIP recombinant protein (Position: K316-N671). Human RIP shares 65% amino acid (aa) sequence identity with mouse RIP.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene. It is mapped to 6p25.2. RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis). The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles. Also, proteolytic processing of RIPk1, through both caspase-dependent and -independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product(s) of RIPk1 upon stress.

Reference :

1. "Entrez Gene: RIPK1 receptor (TNFRSF)-interacting serine-threonine kinase 1" 2. Hsu H, Huang J, Shu HB, Baichwal V, Goeddel DV (June 1996). "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex". Immunity 4 (4): 387–96. 3. Sun, L., Wang, H., Wang, Z., He, S., Chen, S., Liao, D., Wang, L., Yan, J., Liu, W., Lei, X., Wang, X. Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148: 213-227, 2012.

Gene Name :

RIPK1

Protein Name :

Receptor-interacting serine/threonine-protein kinase 1

Gene Full Name :

receptor (TNFRSF)-interacting serine-threonine kinase 1

Synonyms :

Cell death protein RIP antibody FLJ39204 antibody OTTHUMP00000039163 antibody Receptor (TNFRSF) interacting serine threonine kinase 1 antibody receptor interacting protein 1 antibody Receptor interacting protein antibody Receptor interacting serine threonine protein kinase 1 antibody Receptor TNFRSF interacting serine threonine kinase 1 antibody Receptor-interacting protein 1 antibody Receptor-interacting serine/threonine-protein kinase 1 antibody Rinp antibody RIP 1 antibody RIP antibody Rip-1 antibody RIP1 antibody RIPK 1 antibody Ripk1 antibody RIPK1_HUMAN antibody Serine threonine protein kinase RIP antibody Serine/threonine-protein kinase RIP antibody

Uniprot ID :

Q13546

more info or order :

Boster Immunoleader product webpage