Boster Immunoleader

antibody

Anti-Gli1 Antibody

PB9108

100μg/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Gli1 Antibody

PB9108

Polyclonal

Rabbit IgG polyclonal antibody for Zinc finger protein GLI1(GLI1) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100μg/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for GLi1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human GLi1 recombinant protein (Position: Q768-A1106). Human GLi1 shares 79% amino acid (aa) sequence identity with mouse GLi1.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to direct a coherent programme of neurogenesis.

1. Kinzler KW,; Bigner SH; Bigner DD; Trent JM; Law ML; O'Brien SJ; Wong AJ; Vogelstein B. (April 1987). "Identification of an amplified, highly expressed gene in a human glioma". Science 236 (4797): 70–3. 2. Ruiz i Altaba A. (June 1999). "Gli proteins encode context-dependent positive and negative functions: implications for development and disease.". Development 126 (14): 3205–16. 3. Sasaki H,; Hui C; Nakafuku M; Kondoh H. (April 1997). "A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro". Development 124 (7): 1313–22.

GLI1

Zinc finger protein GLI1

GLI family zinc finger 1

Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody

P08151