Anti-GAPDH Antibody | Boster Immunoleader PB9107 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-GAPDH Antibody

catalog :

PB9107

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

publications citing this reagent :

Liu L, Wang P, Liang C, He D, Yu Y, Liu X. Distinct effects of Nampt inhibition on mild and severe models of lipopolysaccharide-induced myocardial impairment. Int Immunopharmacol. 2013;17:342-9 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-GAPDH Antibody

Catalog Number :

PB9107

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) detection. Tested with WB in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Notes :

WB: The detection limit for GAPDH is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human GAPDH(320-335aa YSNRVVDLMAHMASKE), different from the related mouse and rat sequences by one amino acid.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Reference :

1. Bae, B.-I., Hara, M. R., Cascio, M. B., Wellington, C. L., Hayden, M. R., Ross, C. A., Ha, H. C., Li, X.-J., Snyder, S. H., Sawa, A. Mutant Huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Nat. Acad. Sci. 103: 3405-3409, 2006. 2. Colell, A., Ricci, J.-E., Tait, S., Milasta, S., Maurer, U., Bouchier-Hayes, L., Fitzgerald, P., Guio-Carrion, A., Waterhouse, N. J., Li, C. W., Mari, B., Barbry, P., Newmeyer, D. D., Beere, H. M., Green, D. R. GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation. Cell 129: 983-997, 2007. Note: Erratum: Cell 130: 385 only, 2007. 3. Tarze A, Deniaud A, Le Bras M, Maillier E, Molle D, Larochette N, Zamzami N, Jan G, Kroemer G, Brenner C (April 2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization".Oncogene 26 (18): 2606–20.

Gene Name :

GAPDH

Protein Name :

Glyceraldehyde-3-phosphate dehydrogenase

Gene Full Name :

glyceraldehyde-3-phosphate dehydrogenase

Synonyms :

38 kDa BFA-dependent ADP-ribosylation substrate antibody aging associated gene 9 protein antibody Aging-associated gene 9 protein antibody BARS-38 antibody cb609 antibody EC 1.2.1.12 antibody Epididymis secretory sperm binding protein Li 162eP antibody G3P_HUMAN antibody G3PD antibody G3PDH antibody GAPD antibody GAPDH antibody GAPDH antibody Glyceraldehyde 3 phosphate dehydrogenase antibody Glyceraldehyde 3 phosphate dehydrogenase liver antibody Glyceraldehyde 3 phosphate dehydrogenase muscle antibody Glyceraldehyde-3-phosphate dehydrogenase antibody HEL-S-162eP antibody KNC-NDS6 antibody MGC102544 antibody MGC102546 antibody MGC103190 antibody MGC103191 antibody MGC105239 antibody MGC127711 antibody MGC88685 antibody OCAS, p38 component antibodyOCT1 coactivator in S phase, 38-KD component antibody peptidyl cysteine S nitrosylase GAPDH antibody Peptidyl-cysteine S-nitrosylase GAPDH antibody wu:fb33a10 antibody

Uniprot ID :

P04406

more info or order :

Boster Immunoleader product webpage