Boster Immunoleader

antibody

Anti-Desmin Antibody

PB9105

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-Desmin Antibody

PB9105

Polyclonal

Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

WB: The detection limit for Desmin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequences identity with both mouse and rat Desmin.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What’s more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.

1. Muñoz-Mármol AM, Strasser G, Isamat M, Coulombe PA, Yang Y, Roca X, Vela E, Mate JL, Coll J, Fernández-Figueras MT, Navas-Palacios JJ, Ariza A, Fuchs E (September 1998)."A dysfunctional desmin mutation in a patient with severe generalized myopathy". Proc. Natl. Acad. Sci. U.S.A. 95 (19): 11312–7. 2. Li ZL, Lilienbaum A, Butler-Browne G, Paulin D (May 1989). "Human desmin-coding gene: complete nucleotide sequence, characterization and regulation of expression during myogenesis and development". Gene 78 (2): 243–54. 3. Tidball, J. G. Desmin at myotendinous junctions. Exp. Cell Res. 199: 206-212, 1992.

DES

Desmin

desmin

CMD1I antibody CSM1 antibody CSM2 antibody DES antibody DESM_HUMAN antibody Desmin antibody FLJ12025 antibody FLJ39719 antibody FLJ41013 antibody FLJ41793 antibody Intermediate filament protein antibody OTTHUMP00000064865 antibody

P17661