Anti-Cyclin B1 Antibody | Boster Immunoleader PB9104 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cyclin B1 Antibody

catalog :

PB9104

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cyclin B1 Antibody

Catalog Number :

PB9104

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for G2/mitotic-specific cyclin-B1(CCNB) detection. Tested with WB in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Cyclin B1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Reference :

1. Sartor H, Ehlert F, Grzeschik KH, Muller R, Adolph S (Aug 1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively". Genomics 13 (3): 911–2. 2. Kimura K, Hirano M, Kobayashi R, Hirano T (October 1998). "Phosphorylation and activation of 13S condensin by Cdc2 in vitro". Science 282 (5388): 487–90. 3. Heald R, McKeon F (May 1990). "Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis". Cell 61 (4): 579–89.

Gene Name :

CCNB

Protein Name :

G2/mitotic-specific cyclin-B1

Gene Full Name :

cyclin B1

Synonyms :

CCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody

Uniprot ID :

P14635

more info or order :

Boster Immunoleader product webpage