Boster Immunoleader

antibody

Anti-CSK Antibody

PB9102

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-CSK Antibody

PB9102

Polyclonal

Rabbit IgG polyclonal antibody for Tyrosine-protein kinase CSK(CSK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for CSK is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequences identity with both mouse and rat CSK.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene. It is mapped to 15q24.1. CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src. Csk's control of the Src family activity is widely thought to be central to regulation of the immune response. CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus. Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.

1. Bergman M, Mustelin T, Oetken C, Partanen J, Flint NA, Amrein KE, Autero M, Burn P, Alitalo K (August 1992). "The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity. 2. Sun G, Budde RJ (September 1997). "Expression, purification, and initial characterization of human Yes protein tyrosine kinase from a bacterial expression system". Arch. Biochem. Biophys. 345 (1): 135–42. 3. Nada S, Okada M, MacAuley A, Cooper JA, Nakagawa H (May 1991). "Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src". Nature 351 (6321): 69–72.

CSK

Tyrosine-protein kinase CSK

c-src tyrosine kinase

C SRC antibody C SRC kinase antibody C src Tyrosine Kinase antibody C-SRC kinase antibody c-src tyrosine kinase antibody Csk A antibody CSK antibody CSK_HUMAN antibody CYTOPLASMIC TYROSINE KINASE antibody EC 2.7.10.2 antibody MGC112926 antibody MGC117393 antibody MGC154049 antibody P60 Src antibody Protein tyrosine kinase CYL antibody Protein-tyrosine kinase CYL antibody Proto oncogene tyrosine protein kinase antibody Tyrosine protein kinase CSK antibody Tyrosine protein kinase CSK antibody Tyrosine-protein kinase CSK antibody zgc:154049 antibody

P41240