Boster Immunoleader

antibody

Anti-Collagen IV Antibody

PB9099

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-Collagen IV Antibody

PB9099

Polyclonal

Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for Collagen IV is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

1. Soininen R, Haka-Risku T, Prockop DJ, Tryggvason K (Jan 1988). "Complete primary structure of the alpha 1-chain of human basement membrane (type IV) collagen". FEBS Lett 225 (1-2): 188–94. 2. Plaisier E, Gribouval O, Alamowitch S et al. (2007). "COL4A1 mutations and hereditary angiopathy, nephropathy, aneurysms, and muscle cramps". N. Engl. J. Med. 357 (26): 2687–2695. 3. Wang, X., Harris, R. E., Bayston, L. J., Ashe, H. L. Type IV collagens regulate BMP signalling in Drosophila. Nature 455: 72-77, 2008.

COL4A1

Collagen alpha-1(IV) chain

collagen, type IV, alpha 1

Arresten antibody Canstatin antibody CO4A1_HUMAN antibody COL4A1 antibody COL4A1 NC1 domain antibody COL4A2 antibody COL4A3 antibody COL4A4 antibody COL4A5 antibody Collagen Alpha 1(IV) Chain antibody Collagen Alpha 2(IV) Chain antibody collagen alpha-1(IV) chain antibody Collagen IV Alpha 1 Polypeptide antibody Collagen IV Alpha 2 Polypeptide antibody Collagen Of Basement Membrane Alpha 1 Chain antibody Collagen Of Basement Membrane Alpha 2 Chain antibody Collagen Type IV Alpha 1 antibody Collagen Type IV Alpha 2 antibody Collagen Type IV Alpha 3 antibody Collagen Type IV Alpha 4 antibody Collagen Type IV Alpha 5 antibody DKFZp686I14213 antibody FLJ22259 antibody

P02462