Anti-CD45 Antibody | Boster Immunoleader PB9096 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CD45 Antibody

catalog :

PB9096

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Lu Y, Jin X, Chen Y, Li S, Yuan Y, Mai G, et al. Mesenchymal stem cells protect islets from hypoxia/reoxygenation-induced injury. Cell Biochem Funct. 2010;28:637-43 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CD45 Antibody

Catalog Number :

PB9096

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Receptor-type tyrosine-protein phosphatase C(PTPRC) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for CD45 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human CD45 recombinant protein (Position: R1113-S1304). Human CD45 shares 68% amino acid (aa) sequences identity with both mouse and rat CD45.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

CD45(Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Reference :

1. Anderson, J.N. et al. (2004) FASEB J. 18:8. 2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987. 3. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.

Gene Name :

PTPRC

Protein Name :

Receptor-type tyrosine-protein phosphatase C

Gene Full Name :

protein tyrosine phosphatase, receptor type, C

Synonyms :

B220 antibody CD 45 antibody CD45 antibody CD45 antigen antibody CD45R antibody GP180 antibody L CA antibody L-CA antibody LCA antibody Leukocyte common antigen antibody loc antibody Ly-5 antibody LY5 antibody Ly5, homolog of antibody Lyt-4 antibody OTTHUMP00000033813 antibody OTTHUMP00000033816 antibody OTTHUMP00000033817antibody OTTHUMP00000038574 antibody Protein tyrosine phosphatase receptor type C antibody Protein tyrosine phosphatase receptor type c polypeptide antibody protein tyrosine phosphatase, receptor type, C antibody PTPRC antibody PTPRC_HUMAN antibody Receptor-type tyrosine-protein phosphatase C antibody SCID due to PTPRC deficiency antibody T200 antibody T200 glycoprotein antibody T200 leukocyte common antigen antibody

Uniprot ID :

P08575

more info or order :

Boster Immunoleader product webpage