Boster Immunoleader

antibody

Anti-CD43 Antibody

PB9095

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

info changed 20150409

Anti-CD43 Antibody

PB9095

Polyclonal

Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for CD43 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

1. Fukuda M, Carlsson SR (1987). "Leukosialin, a major sialoglycoprotein on human leukocytes as differentiation antigens.". Med. Biol. 64 (6): 335–43. 2. Rogaev EI, Keryanov SA (1993). "Unusual variability of the complex dinucleotide repeat block at the SPN locus.". Hum. Mol. Genet. 1 (8): 657. 3. Rosenstein Y, Park JK, Hahn WC, et al. (1992). "CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1.". Nature 354 (6350): 233–5.

SPN

Leukosialin

sialophorin

CD 43 antibody CD43 antibody CD43 antigen antibody Galactoglycoprotein antibody Galactoglycoprotein antibody GALGP antibody GPL 115 antibody GPL115 antibody Human gene for sialophorin antibody Leucocyte sialoglycoprotein antibody Leucocyte sialoglycoprotein antibody LEUK_HUMAN antibody Leukocyte sialoglycoprotein antibody Leukosialin antibody LSN antibody Sialophorin (leukosialin) antibody Sialophorin antibody SPN antibody

P16150