Anti-Hsp90 Antibody | Boster Immunoleader PB9089 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Hsp90 Antibody

catalog :

PB9089

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Product Name :

Anti-Hsp90 Antibody

Catalog Number :

PB9089

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for Hsp90 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Hsp90 recombinant protein (Position: P2-V365). Human Hsp90 shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

HSP90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. HSP90 inhibitorsare investigated as anti-cancer drugs. Heat shock proteins, as a class, are among the most highly expressedcellular proteins across all species. It is mapped to 14q32.31. Hsp90 acts as a capacitor for morphologic evolution through epigenetic and genetic mechanisms. HSP90 is a molecular chaperone that plays a key role in the conformational maturation of oncogenic signaling proteins, including HER2/ERBB2, AKT, RAF1, BCR-ABL, and mutated p53. Although HSP90 is highly expressed in most cells, HSP90 inhibitors selectively kill cancer cells compared to normal cells, and theHSP90 inhibitor 17-allylaminogeldanamycin (17-AAG) exhibited antitumor activity in preclinical models.

Reference :

1. Csermely P, Schnaider T, Soti C, Prohászka Z, Nardai G (August 1998). "The 90-kDa molecular chaperone family: structure, function, and clinical applications. A comprehensive review". Pharmacol. Ther. 79 (2): 129–68. 2. Sollars, V., Lu, X., Xiao, L., Wang, X., Garfinkel, M. D., Ruden, D. M. Evidence for an epigenetic mechanism by which Hsp90 acts as a capacitor for morphological evolution. Nature Genet. 33: 70-74, 2003. 3. Solit, D. B., Zheng, F. F., Drobnjak, M., Munster, P. N., Higgins, B., Verbel, D., Heller, G., Tong, W., Cordon-Cardo, C., Agus, D. B., Scher, H. I., Rosen, N. 7-Allylamino-17-demethoxygeldanamycin induces the degradation of androgen receptor and HER-2/neu and inhibits the growth of prostate cancer xenografts. Clin. Cancer Res. 8: 986-993, 2002.

Gene Name :

HSP90AA1

Protein Name :

Heat shock protein HSP 90-alpha

Gene Full Name :

heat shock protein 90kDa alpha (cytosolic), class A member 1

Synonyms :

D6S182 antibody FLJ26984 antibody FLJ31884 antibody Heat shock 86 kDa antibody heat shock 90kDa protein 1 alpha antibody Heat shock protein 90kDa alpha cytosolic class A member 1 antibody Heat shock protein 90kDa alpha cytosolic class B member 1 antibody Heat shock protein HSP 90 alpha antibody Heat shock protein HSP 90 beta antibody Heat shock protein HSP 90-alpha antibody HS90A_HUMAN antibody HSP 84 antibody HSP 86 antibody Hsp 90 antibody HSP84 antibody HSP86 antibody Hsp89 antibody Hsp90 antibody HSP90 Beta antibody HSP90A antibody HSP90AA1 antibody HSP90AB1 antibody HSP90B antibody HSP90N antibody HSPC1 antibody HSPC2 antibody HSPCA antibody HSPCAL1 antibody HSPCAL4 antibody HSPCB antibody HSPN antibody LAP2 antibody Lipopolysaccharide associated protein2 antibody LPS associated protein 2 antibody NY REN 38 antigen antibody Renal carcinoma antigen NY-REN-38 antibody

Uniprot ID :

P07900

more info or order :

Boster Immunoleader product webpage