Anti-BDNF Antibody | Boster Immunoleader PB9075 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-BDNF Antibody

catalog :

PB9075

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Wu W, Wang X, Xiang Q, Meng X, Peng Y, Du N, et al. Astaxanthin alleviates brain aging in rats by attenuating oxidative stress and increasing BDNF levels. Food Funct. 2014;5:158-66 pubmed publisher
Li R, Xia W, Zhang Z, Wu K. S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk. PLoS ONE. 2011;6:e21663 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-BDNF Antibody

Catalog Number :

PB9075

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat Predicted to work with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat; Predicted Species: Human

Notes :

WB: The detection limit for BDNF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Reference :

1. Jones, K. R.; Reichardt, L. F. : Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Nat. Acad. Sci. 87: 8060-8064, 1990. 2. Lee, R.; Kermani, P.; Teng, K. K.; Hempstead, B. L. : Regulation of cell survival by secreted proneurotrophins. Science 294: 1945-1948, 2001 . 3. Pruunsild, P.; Kazantseva, A.; Aid, T.; Palm, K.; Timmusk, T. : Dissecting the human BDNF locus: bidirectional transcription, complex splicing, and multiple promoters. Genomics 90: 397-406, 2007.

Gene Name :

BDNF

Protein Name :

Brain-derived neurotrophic factor

Gene Full Name :

brain-derived neurotrophic factor

Synonyms :

Abrineurin antibody BDNF antibody BDNF_HUMAN antibody Brain Derived Neurotrophic Factor antibody Brain-derived neurotrophic factor antibody MGC34632 antibody Neurotrophin antibody

Uniprot ID :

P21237

more info or order :

Boster Immunoleader product webpage