Anti-ALOX15 Antibody | Boster Immunoleader PB9073 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ALOX15 Antibody

catalog :

PB9073

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ALOX15 Antibody

Catalog Number :

PB9073

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Arachidonate 15-lipoxygenase(ALOX15) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for ALOX15 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Reference :

1. Funk CD, Funk LB, FitzGerald GA, Samuelsson B (May 1992)."Characterization of human 12-lipoxygenase genes".Proceedings of the National Academy of Sciences of the United States of America 89 (9): 3962–6. 2. Kelavkar, U. P., Badr, K. F. Effects of mutant p53 expression on human 15-lipoxygenase-promoter activity and murine 12/15-lipoxygenase gene expression: evidence that 15-lipoxygenase is a mutator gene. Proc. Nat. Acad. Sci. 96: 4378-4383, 1999.

Gene Name :

ALOX15

Protein Name :

Arachidonate 15-lipoxygenase

Gene Full Name :

arachidonate 15-lipoxygenase

Synonyms :

15 lipoxygenase 1 antibody 15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody 15 LOX antibody 15 LOX 1 antibody 15-LOX antibody 15LOX 1 antibody ALOX15 antibody Arachidonate 15 lipoxygenase antibody Arachidonate 15-lipoxygenase antibody Arachidonate omega 6 lipoxygenase antibody Arachidonate omega-6 lipoxygenase antibody LOG15 antibody LOX15_HUMAN antibody

Uniprot ID :

P16050

more info or order :

Boster Immunoleader product webpage