Anti-VEGF Antibody | Boster Immunoleader PB9071 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-VEGF Antibody

catalog :

PB9071

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

publications citing this reagent :

Chen Z, Zhang Y, Chen X, Liu X, Wang Z, Zhou X, et al. Retinoic Acid promotes interleukin-4 plasmid-dimethylsulfoxide topical transdermal delivery for treatment of psoriasis. Ann Dermatol. 2015;27:121-7 pubmed publisher
Liu M, Wang Y, Qian C, Li H. Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis. Int J Mol Med. 2014;34:1492-504 pubmed publisher
Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, et al. Therapeutic effects of human amniotic fluid-derived stem cells on renal interstitial fibrosis in a murine model of unilateral ureteral obstruction. PLoS ONE. 2013;8:e65042 pubmed publisher
Huang S, Yang N, Liu Y, Gao J, Huang T, Hu L, et al. Grape seed proanthocyanidins inhibit colon cancer-induced angiogenesis through suppressing the expression of VEGF and Ang1. Int J Mol Med. 2012;30:1410-6 pubmed publisher
Sun D, Wang Y, Liu C, Zhou X, Li X, Xiao A. Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral obstruction. Life Sci. 2012;90:900-9 pubmed publisher
Yan Y, Xu W, Qian H, Zhu W, Mao F, Zhang X. Tumstatin45-132-TNFalpha suppresses tumour growth through anti-angiogenic effects and cytotoxicity. Biotechnol Appl Biochem. 2010;56:119-27 pubmed publisher
Yang W, Zhang Y, Yu J, Li S. The low expression of CD80 correlated with the vascular endothelial growth factor in esophageal cancer tissue. Eur J Surg Oncol. 2010;36:501-6 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-VEGF Antibody

Catalog Number :

PB9071

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for VEGF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Reference :

1. Lee, C. G.; Link, H.; Baluk, P.; Homer, R. J.; Chapoval, S.; Bhandari, V.; Kang, M. J.; Cohn, L.; Kim, Y. K.; McDonald, D. M.; Elias, J. A. : Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. Nature Med. 10: 1095-1103, 2004. 2. Gerber, H.-P.; Malik, A. K.; Solar, G. P.; Sherman, D.; Liang, X. H.; Meng, G.; Hong, K.; Marsters, J. C.; Ferrara, N. : VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. Nature 417: 954-958, 2002. 3. Jin, K.; Zhu, Y.; Sun, Y.; Mao, X. O.; Xie, L.; Greenberg, D. A. : Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo. Proc. Nat. Acad. Sci. 99: 11946-11950, 2002.

Gene Name :

VEGFA

Protein Name :

Vascular endothelial growth factor A

Gene Full Name :

vascular endothelial growth factor A

Synonyms :

MGC70609 antibody MVCD1 antibody Vascular endothelial growth factor A antibody Vascular Endothelial Growth Factor antibody Vascular Permeability Factor antibody VEGF-A antibody VEGFA antibody VEGFA_HUMAN antibody VPF antibody

Uniprot ID :

P15692

more info or order :

Boster Immunoleader product webpage