Anti-P27 KIP 1 Antibody | Boster Immunoleader PB9070 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-P27 KIP 1 Antibody

catalog :

PB9070

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-P27 KIP 1 Antibody

Catalog Number :

PB9070

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cyclin-dependent kinase inhibitor 1B(CDKN1B) detection. Tested with WB in Humnan;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Notes :

WB: The detection limit for P27 KIP 1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Cyclin-dependent kinase inhibitor 1B (p27KIP1), also known as KIP1 or P27, is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kipfamily of cyclin dependent kinase (Cdk) inhibitor proteins. It is mapped to 12p13.1. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of p27KIP1 is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

Reference :

1. Polyak, K., Lee, M.-H., Erdjument-Bromage, H., Koff, A., Roberts, J. M., Tempst, P., Massague, J. Cloning of p27(Kip1), a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78: 59-66, 1994. 2. Hauck, L., Harms, C., An, J., Rohne, J., Gertz, K., Dietz, R., Endres, M., von Harsdorf, R.Protein kinase CK2 links extracellular growth factor signaling with the control of p27(Kip1) stability in the heart. Nature Med. 14: 315-324, 2008. Note: Erratum: Nature Med. 14: 585 only, 2008.

Gene Name :

CDKN1B

Protein Name :

Cyclin-dependent kinase inhibitor 1B

Gene Full Name :

cyclin-dependent kinase inhibitor 1B (p27, Kip1)

Synonyms :

AA408329 antibody AI843786 antibody Cdki1b antibody CDKN 1B antibody CDKN 4 antibody CDKN1B antibody CDKN4 antibody CDN1B_HUMAN antibody Cyclin Dependent Kinase Inhibitor 1B antibody Cyclin dependent kinase inhibitor p27 antibody Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody Cyclin-dependent kinase inhibitor 1B antibody Cyclin-dependent kinase inhibitor p27 antibody Cyclin-dependent kinase inhibitor p27 Kip1 antibody KIP 1 antibody KIP1 antibody MEN1B antibody MEN4 antibody OTTHUMP00000195098 antibody OTTHUMP00000195099 antibody p27 antibody p27 Kip1 antibody P27-like cyclin-dependent kinase inhibitor antibody P27KIP1 antibody

Uniprot ID :

P46527

more info or order :

Boster Immunoleader product webpage