Anti-Fas Ligand Antibody | Boster Immunoleader PB9068 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Fas Ligand Antibody

catalog :

PB9068

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Fas Ligand Antibody

Catalog Number :

PB9068

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Notes :

WB: The detection limit for Fas Ligand is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived mouse Fas Ligand recombinant protein (Position: Q128-L279). Mouse Fas Ligand shares 82% and 93% amino acid (aa) sequences identity with human and rat, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. It is mapped to 1q24.3. FASL may contribute to the immune privilege of tumors. Pharmacologic products that render infiltrating T cells insensitive to FASL-induced killing may break the immunologic unresponsiveness to melanoma and provide a complementary approach in the therapy of malignant melanoma. FASL-mediated apoptosis is important for skin homeostasis, the dysregulation of FAS-FASL interactions may be central to the development of skin cancer. Polymorphisms affecting either the expression or biologic activities of FAS or FASL may contribute to the genetic risk of developing T-cell lymphoblastic lymphomas.

Reference :

1. Hahne, M., Rimoldi, D., Schroter, M., Romero, P., Schreier, M., French, L. E., Schneider, P., Bornand, T., Fontana, A., Lienard, D., Cerottini, J. C., Tschopp, J. Melanoma cell expression of Fas (Apo-1/CD95) ligand: implications for tumor immune escape.Science 274: 1363-1366, 1996. 2. Hill, L. L., Ouhtit, A., Loughlin, S. M., Kripke, M. L., Ananthaswamy, H. N., Owen-Schaub, L. B. Fas ligand: a sensor for DNA damage critical in skin cancer etiology.Science 285: 898-900, 1999. 3. Villa-Morales, M., Santos, J., Perez-Gomez, E., Quintanilla, M., Fernandez-Piqueras, J. A role for the Fas/FasL system in modulating genetic susceptibility to T-cell lymphoblastic lymphomas. Cancer Res. 67: 5107-5116, 2007.

Gene Name :

FASLG

Protein Name :

Tumor necrosis factor ligand superfamily member 6

Gene Full Name :

Fas ligand (TNF superfamily, member 6)

Synonyms :

ALPS1B antibody APO1L antibody Apoptosis (APO 1) antigen ligand 1 antibody Apoptosis antigen ligand 1 antibody Apoptosis antigen ligand antibody APT1LG1 antibody APTL antibody CD178 antibody CD178 antigen antibody CD95 ligand antibody CD95-L antibody CD95L antibody CD95L protein antibody Fas antigen ligand antibody Fas L antibody Fas ligand (TNF superfamily member 6) antibody Fas ligand antibody FASL antibody Fasl Fas ligand (TNF superfamily member 6) antibody FASLG antibody Generalized lymphoproliferative disease antibody Gld antibody soluble form antibody TNF superfamily member 6 antibody TNFL6_HUMAN antibody TNFSF6 antibody Tumor necrosis factor (ligand) superfamily member 6 antibody Tumor necrosis factor ligand superfamily member 6 antibody

Uniprot ID :

P41047

more info or order :

Boster Immunoleader product webpage