Boster Immunoleader

antibody

Anti-CNTF Antibody

PB9067

100 ug/vial

240 USD

polyclonal

rabbit

western blot, ELISA, immunohistochemistry - paraffin section

Anti-CNTF Antibody

PB9067

Polyclonal

Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse, rat

WB,IHC-P,ELISA

Concentration: 0.1-0.5μg/ml; Tested Species: Rat

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Concentration: 0.1-0.5μg/ml; Tested Species: Rat

WB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone andnerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations includingastrocytes. It is mapped to 11q12.1. In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons. CNTF appears not to be involved in motor neuron survival during development. human CNTF has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions. CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.

1. Barbin, G., Manthorpe, M., Varon, S. Purification of the chick eye ciliary neuronotrophic factor. J. Neurochem. 43: 1468-1478, 1984. 2. Raju, S. V. Y., Zheng, M., Schuleri, K. H., Phan, A. C., Bedja, D., Saraiva, R. M., Yiginer, O., Vandegaer, K., Gabrielson, K. L., O'Donnell, C. P., Berkowitz, D. E., Barouch, L. A., Hare, J. M. Activation of the cardiac ciliary neurotrophic factor receptor reverses left ventricular hypertrophy in leptin-deficient and leptin-resistant obesity. Proc. Nat. Acad. Sci. 103: 4222-4227, 2006. 3. Sendtner, M., Schmalbruch, H., Stockli, K. A., Carroll, P., Kreutzberg, G. W., Thoenen, H.Ciliary neurotrophic factor prevents degeneration of motor neurons in mouse mutant progressive motor neuronopathy. Nature 358: 502-504, 1992. 4. Emerich, D. F., Winn, S. R., Hantraye, P. M., Peschanski, M., Chen, E.-Y., Chu, Y., McDermott, P., Baetge, E. E., Kordower, J. H. Protective effect of encapsulated cells producing neurotrophic factor CNTF in a monkey model of Huntington's disease.Nature 386: 395-399, 1997.

CNTF

Ciliary neurotrophic factor

ciliary neurotrophic factor

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P20294