Anti-Wnt5a Antibody | Boster Immunoleader PB9063 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Wnt5a Antibody

catalog :

PB9063

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Wnt5a Antibody

Catalog Number :

PB9063

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Protein Wnt-5a(WNT5A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat Predicted to work with: mouse

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

WB: The detection limit for Wnt5a is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human Wnt5a recombinant protein (Position: D184-D303). Human Wnt5a shares 100% amino acid (aa) sequences identity with both mouse and rat Wnt5a.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. This gene is a member of the WNT gene family. The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation. It is mapped to 3p14.3. WNT5A is highly expressed in the dermal papilla of depilated skin. This protein plays an essential role in regulating developmental pathways during embryogenesis. And this protein may also play a role in oncogenesis. WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.

Reference :

1. "Entrez Gene: WNT5A wingless-type MMTV integration site family, member 5A" 2. Mikels, A. J., Nusse, R. Purified Wnt-5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context. PLoS Biol. 4: e115, 2006. 3. Zhang, X., Zhu, J., Yang, G.-Y., Wang, Q.-J., Qian, L., Chen, Y.-M., Chen, F., Tao, Y., Hu, H.-S., Wang, T., Luo, Z.-G. Dishevelled promotes axon differentiation by regulating atypical protein kinase C. Nature Cell Biol. 9: 743-754, 2007.

Gene Name :

WNT5A

Protein Name :

Protein Wnt-5a

Gene Full Name :

wingless-type MMTV integration site family, member 5A

Synonyms :

hWNT 5A antibody hWNT5A antibody Protein Wnt 5a antibody Protein Wnt-5a antibody Protein Wnt5a antibody Wingless type MMTV integration site family member 5A antibody Wnt 5a antibody WNT 5A protein antibody WNT 5A protein precursor antibody WNT5A antibody WNT5A protein precursor antibody WNT5A_HUMAN antibody

Uniprot ID :

P41221

more info or order :

Boster Immunoleader product webpage