Boster Immunoleader

antibody

Anti-CD10 Antibody

PB9058

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-CD10 Antibody

PB9058

Polyclonal

Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for CD10 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

1. Barker, P. E., Shipp, M. A., D'Adamio, L., Masteller, E. L., Reinherz, E. L. The common acute lymphoblastic leukemia antigen gene maps to chromosomal region 3(q21-q27). J. Immun. 142: 283-287, 1989. 2. Northridge, D. B., Jardine, A. G., Alabaster, C. T., Barclay, P. L., Connell, J. M. C., Dargie, H. J., Dilly, S. G., Findlay, I. N., Lever, A. F., Samuels, G. M. R. Effects of UK 69 578: a novel atriopeptidase inhibitor. Lancet 332: 591-593, 1989. Note: Originally Volume II. 3. Stephenson, S. L., Kenny, A. J. The hydrolysis of human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11. Biochem. J. 243: 183-187, 1987. 4 Tran-Paterson, R., Willard, H. F., Letarte, M. The common acute lymphoblastic leukemia antigen (neutral endopeptidase--3.4.24.11) gene is located on human chromosome 3. Cancer Genet. Cytogenet. 42: 129-134, 1989.

MME

Neprilysin

membrane metallo-endopeptidase

Atriopeptidase antibody CALLA antibody CD10 antibody CD10 antigen antibody Common acute lymphocytic leukemia antigen antibody DKFZp686O16152 antibody EC 3.4.24.11 antibody Enkephalinase antibody EPN antibody gp100 antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody Membrane metallo endopeptidase antibody Membrane metallo endopeptidase variant 1 antibody Membrane metallo endopeptidase variant 2 antibody Membrane metalloendopeptidase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody Membrane metalloendopeptidase variant 1 antibody Membrane metalloendopeptidase variant 2 antibody MGC126681 antibody MGC126707 antibody MME antibody NEP antibody NEP_HUMAN antibody Neprilysin antibody Neutral endopeptidase 24.11 antibody Neutral endopeptidase antibody SFE antibody Skin fibroblast elastase antibody

P08473