Boster Immunoleader

antibody

Anti-CD20 Antibody

PB9050

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

Anti-CD20 Antibody

PB9050

Polyclonal

Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P in Human.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

WB: The detection limit for CD20 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What’s more, this protein may be involved in the regulation of B-cell activation and proliferation.

1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005). "The biology of CD20 and its potential as a target for mAb therapy". Curr. Dir. Autoimmun. Current Directions in Autoimmunity 8: 140–74. 2. Hardy, Richard (2008). "Chapter 7: B Lymphocyte Development and Biology". In Paul, William.Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 237–269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010). "CD20 deficiency in humans results in impaired T cell-independent antibody responses". J. Clin. Invest. 120 (1): 214–22.

MS4A1

B-lymphocyte antigen CD20

membrane-spanning 4-domains, subfamily A, member 1

APY antibody ATOPY antibody B lymphocyte antigen CD20 antibody B Lymphocyte Cell Surface Antigen B1 antibody B-lymphocyte antigen CD20 antibody B-lymphocyte cell-surface antigen B1 antibody B-lymphocyte surface antigen B1 antibody B1 antibody Bp 35 antibody Bp35 antibody CD 20 antibody CD20 antibody CD20 antigen antibody CD20 receptor antibody CD20_HUMAN antibody Fc epsilon receptor I beta chain antibody Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody FCER1B antibody FCERI antibody High affinity immunoglobulin epsilon receptor subunit beta antibody IgE Fc receptor subunit beta antibody IGEL antibody IGER antibody IGHER antibody LEU16 antibody LEU16 antibody leukocyte surface antigen Leu 16 antibody Leukocyte surface antigen Leu-16 antibody Ly44 antibody Membrane spanning 4 domains subfamily A member 2 antibody Membrane-spanning 4-domains subfamily A member 1 antibody MS4A1 antibody

P11836