Anti-Cathepsin D Antibody | Boster Immunoleader PB9048 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cathepsin D Antibody

catalog :

PB9048

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cathepsin D Antibody

Catalog Number :

PB9048

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cathepsin D(CTSD) detection. Tested with WB, IHC-P in Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Notes :

WB: The detection limit for Cathepsin D is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Reference :

1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985). "Cloning and sequence analysis of cDNA for human cathepsin D". Proc Natl Acad Sci U S A 82 (15): 4910–4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003). "Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer". Am. J. Pathol. 162 (4): 1183–90.

Gene Name :

CTSD

Protein Name :

Cathepsin D

Gene Full Name :

cathepsin D

Synonyms :

CatD antibody CATD_HUMAN antibody Cathepsin D heavy chain antibody Cathepsin D light chain antibody CathepsinD antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody CTSD antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody

Uniprot ID :

P18242

more info or order :

Boster Immunoleader product webpage