Boster Immunoleader

antibody

Anti-Calbindin Antibody

PB9045

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Calbindin Antibody

PB9045

Polyclonal

Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

WB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

1. Parmentier M.; "The human calbindins: cDNA and gene cloning.";Adv. Exp. Med. Biol. 255:233-240(1989). 2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.;"The mouse calretinin gene promoter region: structural and functional components.";Brain Res. Mol. Brain Res. 49:175-187(1997). 3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.;"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.";Biochemistry 30:656-662(1991).

CALB1

Calbindin

calbindin 1, 28kDa

avian-type antibody CAB27 antibody CALB 1 antibody CALB antibody CALB1 antibody CALB1_HUMAN antibody Calbindin 1 28kDa antibody Calbindin antibody Calbindin D28 antibody D 28K antibody D-28K antibody D28K antibody OTTHUMP00000166027 antibody OTTHUMP00000225441 antibody RTVL H protein antibody Vitamin D dependent calcium binding protein antibody Vitamin D dependent calcium binding protein avian type antibody Vitamin D dependent calcium binding protein avian-type antibody Vitamin D-dependent calcium-binding protein antibody

P05937