Boster Immunoleader

antibody

Anti-Annexin V Antibody

PB9044

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-Annexin V Antibody

PB9044

Polyclonal

Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Rat

WB: The detection limit for Annexin V is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

1. Bogdanova, N., Horst, J., Chlystun, M., Croucher, P. J. P., Nebel, A., Bohring, A., Todorova, A., Schreiber, S., Gerke, V., Krawczak, M., Markoff, A. A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss.Hum. Molec. Genet. 16: 573-578, 2007. 2. Demange, P., Voges, D., Benz, J., Liemann, S., Gottig, P., Berendes, R., Burger, A., Huber, R. Annexin V: the key to understanding ion selectivity and voltage regulation?Trends Biochem. Sci. 19: 272-276, 1994. 3. Sofiadis A, Becker S, Hellman U, Hultin-Rosenberg L, Dinets A, Hulchiy M, Zedenius J, Wallin G, Foukakis T, Höög A, Auer G, Lehtiö J, Larsson C. (April 2012). "Proteomic profiling of follicular and papillary thyroid tumors.". Eur J Endocrinol. 166 (4): 657–67.

ANXA5

Annexin A5

annexin A5

Anchorin CII antibody Annexin 5 antibody Annexin A5 antibody Annexin V antibody Annexin-5 antibody Annexin5 antibody AnnexinA5 antibody AnnexinV antibody ANX 5 antibody ANX A5 antibody ANX5 antibody ANXA5 antibody ANXA5_HUMAN antibody Calphobindin I antibody CBP I antibody CBP-I antibody Endonexin II antibody ENX 2 antibody ENX2 antibody Lipocortin V antibody PAP I antibody PAP-I antibody Placental anticoagulant protein 4 antibody Placental anticoagulant protein I antibody PLACENTAL PROTEIN 4 antibody PP 4 antibody PP4 antibody Thromboplastin inhibitor antibody VAC alpha antibody VAC-alpha antibody Vascular anticoagulant alpha antibody Vascular anticoagulant-alpha antibody

P08758