Boster Immunoleader

antibody

Anti-AKT2 Antibody

PB9043

100 ug/vial

240 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-AKT2 Antibody

PB9043

Polyclonal

Rabbit IgG polyclonal antibody for RAC-beta serine/threonine-protein kinase(AKT2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, mouse, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Human

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

WB: The detection limit for AKT2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2(454-481aa DRYDSLGLLELDQRTHFPQFSYSASIRE), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.

1. Cheng, J. Q., Ruggeri, B., Klein, W. M., Sonoda, G., Altomare, D. A., Watson, D. K., Testa, J. R. Amplification of AKT2 in human pancreatic cancer cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA. Proc. Nat. Acad. Sci. 93: 3636-3641, 1996. 2. Li, X., Monks, B., Ge, Q., Birnbaum, M. J. Akt/PKB regulates hepatic metabolism by directly inhibiting PGC-1-alpha transcription coactivator. Nature 447: 1012-1016, 2007.

AKT2

RAC-beta serine/threonine-protein kinase

v-akt murine thymoma viral oncogene homolog 2

AKT 2 antibody Akt2 antibody AKT2 protein antibody AKT2_HUMAN antibody HIHGHH antibody Murine thymoma viral (v akt) homolog 2 antibody murine thymoma viral (v-akt) homolog-2 antibody PKB antibody PKB beta antibody PKBB antibody PKBbeta antibody PRKBB antibody Protein kinase Akt 2 antibody Protein kinase Akt-2 antibody Protein kinase B beta antibody RAC beta antibody RAC beta serine threonine protein kinase antibody RAC PK beta antibody Rac protein kinase beta antibody RAC-BETA antibody RAC-beta serine/threonine-protein kinase antibody RAC-PK-beta antibody RACbeta antibody V akt murine thymoma viral oncogene homolog 2 antibody

P31751