Anti-LIF Antibody | Boster Immunoleader PB9036 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-LIF Antibody

catalog :

PB9036

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-LIF Antibody

Catalog Number :

PB9036

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

WB: The detection limit for LIF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Reference :

1. Bamberger, A.-M., Jenatschke, S., Schulte, H. M., Loning, T., Bamberger, C. M. Leukemia inhibitory factor (LIF) stimulates the human HLA-G promoter in JEG3 choriocarcinoma cells. J. Clin. Endocr. Metab. 85: 3932-3936, 2000. 2. Sutherland, G. R., Baker, E., Hyland, V. J., Callen, D. F., Stahl, J., Gough, N. M. The gene for human leukemia inhibitory factor (LIF) maps to 22q12. Leukemia 3: 9-13, 1989.

Gene Name :

LIF

Protein Name :

Leukemia inhibitory factor

Gene Full Name :

leukemia inhibitory factor

Synonyms :

CDF antibody Cholinergic Differentiation Factor antibody D factor antibody DIA antibody Differentiation inducing factor antibody differentiation inhibitory activity antibody Differentiation stimulating factor antibody Differentiation-stimulating factor antibody Emfilermin antibody Hepatocyte stimulating factor III antibody HILDA antibody Human interleukin in DA cells antibody Leukemia inhibitory factor antibody LIF antibody LIF_HUMAN antibody Melanoma derived LPL inhibitor antibody Melanoma-derived LPL inhibitor antibody MLPLI antibody

Uniprot ID :

P15018

more info or order :

Boster Immunoleader product webpage