Anti-CNTF Antibody | Boster Immunoleader PB9032 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-CNTF Antibody

catalog :

PB9032

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-CNTF Antibody

Catalog Number :

PB9032

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P in Mouse;Rat.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Mouse, Rat

Notes :

WB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived mouse CNTF recombinant protein (Position: A2-M198). Mouse CNTF shares 83% and 95% amino acid (aa) sequences identity with human and rat CNTF, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The mouse CNTF gene is on mouse chromosome 19 and that its expression is unaffected in the mouse neurologic mutant wobbler, a form of spinal muscular atrophy. The CNTF protein is highly conserved in evolution. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

Reference :

1. Lam, A., Fuller, F., Miller, J., Kloss, J., Manthorpe, M., Varon, S., Cordell, B. Sequence and structural organization of the human gene encoding ciliary neurotrophic factor. Gene 102: 271-276, 1991. 2. Watt, M. J., Dzamko, N., Thomas, W. G., Rose-John, S., Ernst, M., Carling, D., Kemp, B. E., Febbraio, M. A., Steinberg, G. R. CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK. Nature Med. 12: 541-548, 2006.

Gene Name :

CNTF

Protein Name :

Ciliary neurotrophic factor

Gene Full Name :

ciliary neurotrophic factor

Synonyms :

Ciliary Neurotrophic Factor antibody CNTF antibody CNTF_HUMAN antibody HCNTF antibody

Uniprot ID :

P51642

more info or order :

Boster Immunoleader product webpage