Anti-P53 Antibody | Boster Immunoleader PB9008 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-P53 Antibody

catalog :

PB9008

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-P53 Antibody

Catalog Number :

PB9008

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cellular tumor antigen p53(TP53) detection. Tested with WB, IHC-P in Human.

Price :

240 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

E.coli-derived human P53 recombinant protein (Position: A74-D393). Human P53 shares 83% and 85% amino acid (aa) sequences identity with mouse and rat P53, respectively.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Reference :

1. Isobe, M.; Emanuel, B. S.; Givol, D.; Oren, M.; Croce, C. M. : Localization of gene for human p53 tumour antigen to band 17p13. Nature 320: 84-85, 1986. 2. Casey, G.; Yamanaka, Y.; Freiss, H.; Kobrin, M. S.; Lopez, M. E.; Buchler, M.; Beger, H. G.; Korc, M. : p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. Cancer Lett. 69: 151-160, 1993.

Gene Name :

TP53

Protein Name :

Cellular tumor antigen p53

Gene Full Name :

tumor protein p53

Synonyms :

Antigen NY-CO-13 antibody BCC7 antibody Cellular tumor antigen p53 antibody Cys 51 Stop antibody FLJ92943 antibody HGNC11998 antibody LFS1 antibody Mutant tumor protein 53 antibody p53 antibody p53 Cellular Tumor Antigen antibody p53 tumor suppressor antibody P53_HUMAN antibody Phosphoprotein p53 antibody Tp53 antibody Transformation related protein 53 antibody TRP53 antibody Tumor protein p53 antibody Tumor suppressor p53 antibody Tumour Protein p53 antibody

Uniprot ID :

P04637

more info or order :

Boster Immunoleader product webpage