Boster Immunoleader

antibody

Anti-GM-CSF Antibody

PB9003

100 ug/vial

240 USD

polyclonal

rabbit

western blot, immunohistochemistry - paraffin section

info changed 20140731

Anti-GM-CSF Antibody

PB9003

Polyclonal

Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.

240 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Mouse

WB: The detection limit for GM-CSF is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

1. Le Beau, M. M., Espinosa, R., III, Neuman, W. L., Stock, W., Roulston, D., Larson, R. A., Keinanen, M., Westbrook, C. A.Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 2. Park, B. K., Zhang, H., Zeng, Q., Dai, J., Keller, E. T., Giordano, T., Gu, K., Shah, V., Pei, L., Zarbo, R. J., McCauley, L., Shi, S., Chen, S., Wang, C.-Y.NF-kappa-B in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GM-CSF.Nature Med. 13: 62-69, 2007. 3. Uchida, K., Beck, D. C., Yamamoto, T., Berclaz, P.-Y., Abe, S., Staudt, M. K., Carey, B. C., Filippi, M.-D., Wert, S. E., Denson, L. A., Puchalski, J. T., Hauck, D. M., Trapnell, B. C.GM-CSF autoantibodies and neutrophil dysfunction in pulmonary alveolar proteinosis.New Eng. J. Med. 356: 567-579, 2007.

CSF2

Granulocyte-macrophage colony-stimulating factor

colony stimulating factor 2 (granulocyte-macrophage)

Burst Promoting Activity antibody CMCSF antibody Colony stimulating factor 2 (granulocyte-macrophage) antibody Colony Stimulating Factor 2 antibody Colony stimulating factor antibody Colony-stimulating factor antibody CSF 2 antibody CSF Alpha antibody CSF antibody CSF2 antibody CSF2_HUMAN antibody Eosinophil Colony Stimulating Factor antibody GM-CSF antibody GMCSF antibody Granulocyte Macrophage Colony Stimulating Factor antibody Granulocyte-macrophage colony-stimulating factor antibody MGC131935 antibody MGC138897 antibody Molgramostin antibody Pluripoietin Alpha antibody Sargramostim antibody

P01587