Anti-SP100 Antibody | Boster Immunoleader PA2286 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-SP100 Antibody

catalog :

PA2286

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-SP100 Antibody

Catalog Number :

PA2286

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Nuclear autoantigen Sp-100(SP100) detection. Tested with WB in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human SP100(664-677aa YTLKVLMENKFLPE).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

SP100, also known as LYSP100B, INCLUDED, encodes a subnuclear organelle and major component of the PML(promyelocytic leukemia)-SP100 nuclear bodies. SP100 and PML are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is though to play a role in tumorigenesis, immunity, and gene regulation. It has been found that HP1 expression is enhanced when SP100 synthesis is induced by interferon. SP100 can function as a transcriptional coactivator of ETS1 and ETS2, and under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA.

Reference :

1. Seeler, J. S., Marchio, A., Sitterlin, D., Transy, C., Dejean, A. Interaction of SP100 with HP1 proteins: a link between the promyelocytic leukemia-associated nuclear bodies and the chromatin compartment. Proc. Nat. Acad. Sci. 95: 7316-7321, 1998. 2. Szostecki, C., Guldner, H. H., Netter, H. J., Will, H. Isolation and characterization of cDNA encoding a human nuclear antigen predominantly recognized by autoantibodies from patients with primary biliary cirrhosis. J. Immun. 145: 4338-4347, 1990.

Gene Name :

SP100

Protein Name :

Nuclear autoantigen Sp-100

Gene Full Name :

SP100 nuclear antigen

Synonyms :

DKFZp686E07254 antibody FLJ00340 antibody FLJ34579 antibody K297 antibody Lysp100b antibody MYL antibody Nuclear antigen Sp100 antibody Nuclear autoantigen Sp 100 antibody Nuclear autoantigen Sp-100 antibody Nuclear autoantigen Sp100 antibody Nuclear dot associated Sp100 protein antibody Nuclear dot-associated Sp100 protein antibody PP8675 antibody Probable transcription factor PML antibody Promyelocytic leukemia inducer of antibody RING finger protein 71 antibody RNF71 antibody SP 100 antibody SP100 antibody SP100 HMG nuclear autoantigen antibody SP100 nuclear antigen antibody Speckled 100 kDa antibody TRIM19 antibody Tripartite motif protein TRIM19 antibody

Uniprot ID :

P23497

more info or order :

Boster Immunoleader product webpage