Anti-ATP7b Antibody | Boster Immunoleader PA2262 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-ATP7b Antibody

catalog :

PA2262

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-ATP7b Antibody

Catalog Number :

PA2262

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Copper-transporting ATPase 2(ATP7B) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human ATP7b(1451-1465aa DKWSLLLNGRDEEQYI), different from the related mouse and rat sequences by three amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

ATPase, Cu++ transporting, beta polypeptide(Wilson disease) protein, also called ATP7B, is an ATPase that transports copper. This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two putative copper-binding sites. ATP7B is mapped to 13q14.3. This protein functions as a monomer, exporting copper out of the cells. When copper levels are in excess, ATP7B redistributes to a vesicular compartment near the biliary canalicular membranes for elimination of excess copper into bile, and it is transported along liver cell microtubules via interaction with the p62 dynactin subunit.

Reference :

1. "Entrez Gene: ATP7B ATPase, Cu++ transporting, beta polypeptide" 2. Lim, C. M., Cater, M. A., Mercer, J. F. B., La Fontaine, S. Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A. J. Biol. Chem. 281: 14006-14014, 2006.

Gene Name :

ATP7B

Protein Name :

Copper-transporting ATPase 2

Gene Full Name :

ATPase, Cu++ transporting, beta polypeptide

Synonyms :

ATP7B antibody ATPase Cu++ transporting beta polypeptide antibody ATPase Cu++ transporting beta polypeptide antibody ATPase, Cu(2+) transporting, beta polypeptide antibody ATPase, Cu++ transporting, beta polypeptide antibody Copper pump 2 antibody Copper transporting ATPase 2 antibody PWD antibody Toxic milk antibody tx antibody WC1 antibody WD antibody Wilson disease associated protein antibody Wilson disease-associated protein antibody WND antibody WND/140 kDa antibody

Uniprot ID :

P35670

more info or order :

Boster Immunoleader product webpage