Boster Immunoleader

antibody

Anti-Apg7 Antibody

PA2261

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-Apg7 Antibody

PA2261

Polyclonal

Rabbit IgG polyclonal antibody for Ubiquitin-like modifier-activating enzyme ATG7(ATG7) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7(652-669aa KVLDQYEREGFNFLAKVF), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

1. "Entrez Gene: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae)" 2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Note: Erratum: Science 341: 457 only, 2013. 3. Tanida, I., Tanida-Miyake, T., Ueno, T., Kominami, E. The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem. 276: 1701-1706, 2001.

ATG7

Ubiquitin-like modifier-activating enzyme ATG7

autophagy related 7

1810013K23Rik antibody Apg 7 antibody APG7 autophagy 7 like antibody APG7 autophagy 7-like(S. cerevisiae) antibody APG7 like antibody APG7, S. cerevisiae, homolog of antibody APG7-like antibody APG7L antibody ATG 7 antibody ATG12-activating enzyme E1 ATG7 antibody atg7 antibody ATG7 autophagy related 7 homolog(S. cerevisiae) antibody ATG7 autophagy related 7 homolog antibody Atg7l antibody Autophagy 7, S. cerevisiae, homolog of antibody Autophagy related protein 7 antibody Autophagy-related 7(yeast) antibody Autophagy-related protein 7 antibody DKFZp434N0735 antibody GSA 7 antibody GSA7 antibody hAGP7 antibody Ubiquitin activating enzyme E1 like protein antibody Ubiquitin-activating enzyme E1-like protein antibody Ubiquitin-like modifier-activating enzyme ATG7 antibody

O95352