Boster Immunoleader

antibody

Anti-UBE2I/UBC9 Antibody

PA2258

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-UBE2I/UBC9 Antibody

PA2258

Polyclonal

Rabbit IgG polyclonal antibody for SUMO-conjugating enzyme UBC9(UBE2I) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: rat Predicted to work with: human, mouse

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human UBE2I(108-122aa IKQILLGIQELLNE), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

SUMO-conjugating enzyme UBC9(UBE2I), also called UBC9, is a protein that in humans is encoded by the UBE2I gene. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. It is mapped to 16p13.3. UBC9 could fully complement the mutant phenotype of a yeast ubc9 mutant strain. This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells. What's more, it could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9. UBC9 is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBC9.

1. Shi, Y., Zou, M., Farid, N. R., Paterson, M. C. Association of FHIT (fragile histidine triad), a candidate tumour suppressor gene, with the ubiquitin-conjugating enzyme hUBC9. Biochem. J. 352: 443-448, 2000. 2. Wang, Z.-Y., Qiu, Q.-Q., Seufert, W., Taguchi, T., Testa, J. R., Whitmore, S. A., Callen, D. F., Welsh, D., Shenk, T., Deuel, T. F. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. 3. Watanabe TK, Fujiwara T, Kawai A, Shimizu F, Takami S, Hirano H, Okuno S, Ozaki K, Takeda S, Shimada Y, Nagata M, Takaichi A, Takahashi E, Nakamura Y, Shin S (March 1996). "Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle".Cytogenet Cell Genet 72 (1): 86–9.

UBE2I

SUMO-conjugating enzyme UBC9

ubiquitin-conjugating enzyme E2I

C358B7.1 antibody Homologous to yeast UBC9 antibody p18 antibody SUMO 1 conjugating enzyme antibody SUMO 1 protein ligase antibody SUMO conjugating enzyme UBC9 antibody SUMO protein ligase antibody SUMO-conjugating enzyme UBC9 antibody SUMO-protein ligase antibody SUMO1 conjugating enzyme antibody SUMO1 protein ligase antibody UBC 9 antibody UBC9 antibody UBC9 homolog antibody UBC9_HUMAN antibody UBCE 9 antibody UBCE9 antibody UBE 2I antibody Ube2i antibody Ubiquitin carrier protein 9 antibody Ubiquitin carrier protein antibody Ubiquitin carrier protein I antibody Ubiquitin conjugating enzyme 9 antibody Ubiquitin conjugating enzyme E2I(homologous to yeast UBC9) antibody Ubiquitin conjugating enzyme E2I(UBC9 homolog, yeast) antibody Ubiquitin conjugating enzyme E2I antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin like protein SUMO1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin protein ligase I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody

P63279