Anti-RTN3 Antibody | Boster Immunoleader PA2256 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-RTN3 Antibody

catalog :

PA2256

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-RTN3 Antibody

Catalog Number :

PA2256

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Reticulon-3(RTN3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human RTN3(1017-1032aa EKIQAKLPGIAKKKAE), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Background :

Reticulon-3, also known as RTN3 or NSPL2, is a protein that in humans is encoded by the RTN3 gene. It is mapped to 11q13, which is between markers D11S4535 and D11S4627. This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1(BACE1), and the production of amyloid-beta. RTN3 colocalized with BACE1 in neurons of human brain gray matter, and overexpression of RTN3 in vitro inhibited BACE1 activity and decreased amyloid precursor protein processing.

Reference :

1. "Entrez Gene: RTN3 reticulon 3" 2. He, W., Lu, Y., Qahwash, I., Hu, X.-Y., Chang, A., Yan, R. Reticulon family members modulate BACE1 activity and amyloid-beta peptide generation. Nature Med. 10: 959-965, 2004. 3. Moreira, E. F., Jaworski, C. J., Rodriguez, I. R. Cloning of a novel member of the reticulon gene family (RTN3): gene structure and chromosomal localization to 11q13.Genomics 58: 73-81, 1999.

Gene Name :

RTN3

Protein Name :

Reticulon-3

Gene Full Name :

reticulon 3

Synonyms :

ASYIP antibody Neuroendocrine specific protein like 2 antibody Neuroendocrine-specific protein-like 2 antibody Neuroendocrine-specific protein-like II antibody NSP like protein II antibody NSP-like protein 2 antibody NSP-like protein II antibody NSPL2 antibody NSPLII antibody Reticulon 3 antibody Reticulon-3 antibody Reticulon3 antibody Rtn3 antibody RTN3_HUMAN antibody

Uniprot ID :

O95197

more info or order :

Boster Immunoleader product webpage