Anti-IRF1 Antibody | Boster Immunoleader PA2237 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-IRF1 Antibody

catalog :

PA2237

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-IRF1 Antibody

Catalog Number :

PA2237

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Interferon regulatory factor 1(IRF1) detection. Tested with WB, IHC-P, ICC in Human.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human IRF1(169-183aa YMQDLEVEQALTPAL).

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

Interferon regulatory factor 1, also known as MAR is a protein that in humans is encoded by the IRF1 gene. The IRF1 gene is mapped to chromosome 5q31.1 by pulsed-field gel electrophoresis. IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor(IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.

Reference :

1. Ko, J., Gendron-Fitzpatrick, A., Splitter, G. A. Susceptibility of IFN regulatory factor-1 and IFN consensus sequence binding protein-deficient mice to brucellosis. J. Immun. 168: 2433-2440, 2002. 2. Wessely, R., Hengst, L., Jaschke, B., Wegener, F., Richter, T., Lupetti, R., Paschalidis, M., Schomig, A., Brandl, R., Neumann, F.-J. A central role of interferon regulatory factor-1 for the limitation of neointimal hyperplasia. Hum. Molec. Genet. 12: 177-187, 2003.

Gene Name :

IRF1

Protein Name :

Interferon regulatory factor 1

Gene Full Name :

interferon regulatory factor 1

Synonyms :

MAR antibody IRF-1 antibody interferon regulatory factor-1 antibody

Uniprot ID :

P10914

more info or order :

Boster Immunoleader product webpage