Boster Immunoleader

antibody

Anti-E3 SUMO-protein ligase PIAS4 Antibody

PA2215

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

Anti-E3 SUMO-protein ligase PIAS4 Antibody

PA2215

Polyclonal

Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS4(PIAS4) detection. Tested with WB in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human Predicted to work with: mouse, rat

WB

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human E3 SUMO-protein ligase PIAS4(295-310aa HPELCKALVKEKLRLD), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

E3 SUMO-protein ligase PIAS4, also known as protein inhibitor of activated STAT protein 4(PIAS4) or protein inhibitor of activated STAT protein gamma(PIASg or PIASy) is an enzyme that in humans is encoded by the PIRAS4 gene. This gene is mapped to 19p13.3. This gene plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. It functions as an E3-type small ubiquitin-like modifier(SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This gene involved in gene silencing.

1. Bischof, O., Schwamborn, K., Martin, N., Werner, A., Sustmann, C., Grosschedl, R., Dejean, A. The E3 SUMO ligase PIASy is a regulator of cellular senescence and apoptosis. Molec. Cell 22: 783-794, 2006. 2. Galanty, Y., Belotserkovskaya, R., Coates, J., Polo, S., Miller, K. M., Jackson, S. P. Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature 462: 935-939, 2009. 3. Sachdev, S., Bruhn, L., Sieber, H., Pichler, A., Melchior, F., Grosschedl, R. PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies. Genes Dev. 15: 3088-3103, 2001.

PIAS4

E3 SUMO-protein ligase PIAS4

protein inhibitor of activated STAT, 4

E3 SUMO protein ligase PIAS4 antibody E3 SUMO-protein ligase PIAS4 antibody FLJ12419 antibody MGC35296 antibody PAIASgamma antibody PIAS 4 antibody PIAS gamma antibody PIAS-gamma antibody PIAS4 antibody PIAS4_HUMAN antibody PIASG antibody PIASgamma antibody PIASy antibody Protein inhibitor of activated STAT 4 antibody Protein inhibitor of activated STAT protein 4 antibody Protein inhibitor of activated STAT protein gamma antibody Protein inhibitor of activated STAT protein PIASy antibody Zinc finger MIZ type containing 6 antibody ZMIZ 6 antibody ZMIZ6 antibody

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