Anti-AKT3 Antibody | Boster Immunoleader PA2198 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-AKT3 Antibody

catalog :

PA2198

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

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product information

Availability :

info changed 20150327

Product Name :

Anti-AKT3 Antibody

Catalog Number :

PA2198

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for RAC-gamma serine/threonine-protein kinase(AKT3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, mouse, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Mouse; Predicted Species: Human, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human AKT3(122-136aa TSQIDNIGEEEMDAS), identical to the related mouse and rat sequences.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Reference :

1. Boland, E., Clayton-Smith, J., Woo, V. G., McKee, S., Manson, F. D. C., Medne, L., Zackai, E., Swanson, E. A., Fitzpatrick, D., Millen, K. J., Sherr, E. H., Dobyns, W. B., Black, G. C. M. Mapping of deletion and translocation breakpoints in 1q44 implicates the serine/threonine kinase AKT3 in postnatal microcephaly and agenesis of the corpus callosum. Am. J. Hum. Genet. 81: 292-303, 2007. 2. Poduri, A., Evrony, G. D., Cai, X., Elhosary, P. C., Beroukhim, R., Lehtinen, M. K., Hills, L. B., Heinzen, E. L., Hill, A., Hill, R. S., Barry, B. J., Bourgeois, B. F. D., Riviello, J. J., Barkovich, A. J., Black, P. M., Ligon, K. L., Walsh, C. A. Somatic activation of AKT3 causes hemispheric developmental brain malformations. Neuron 74: 41-48, 2012.

Gene Name :

AKT3

Protein Name :

RAC-gamma serine/threonine-protein kinase

Gene Full Name :

v-akt murine thymoma viral oncogene homolog 3

Synonyms :

Akt3 antibody AKT3 kinase antibody AKT3_HUMAN antibody DKFZp434N0250 antibody PKB gamma antibody PKBG antibody PRKBG antibody Protein kinase Akt-3 antibody Protein Kinase AKT3 antibody Protein kinase B gamma antibody RAC gamma antibody RAC gamma serine/threonine protein kinase antibody RAC-gamma serine/threonine-protein kinase antibody RAC-PK-gamma antibody RACPK Gamma antibody Serine threonine protein kinase Akt 3 antibody Serine threonine protein kinase Akt3 antibody STK 2 antibody STK-2 antibody STK2 antibody V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody

Uniprot ID :

Q9Y243

more info or order :

Boster Immunoleader product webpage