Boster Immunoleader

antibody

Anti-SLC12A3 Antibody

PA2170

100 ug/vial

200 USD

polyclonal

rabbit

human, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-SLC12A3 Antibody

PA2170

Polyclonal

Rabbit IgG polyclonal antibody for Solute carrier family 12 member 3(SLC12A3) detection. Tested with WB, IHC-P, IHC-F in Human;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A3(310-328aa EDKASKGFFSYRADIFVQN), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Solute carrier family 12(sodium/chloride transporters), member 3, also called NCCT or TSC, encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. By fluorescence in situ hybridization, this gene is mapped to 16q13. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure.

1. Glaudemans, B., Yntema, H. G., San-Cristobal, P., Schoots, J., Pfundt, R., Kamsteeg, E.-J., Bindels, R. J., Knoers, N. V. A. M., Hoenderop, J. G., Hoefsloot, L. H. Novel NCC mutants and functional analysis in a new cohort of patients with Gitelman syndrome. Europ. J. Hum. Genet. 20: 263-270, 2012. 2. Yang, S.-S., Lo, Y.-F., Yu, I.-S., Lin, S.-W., Chang, T.-H., Hsu, Y.-J., Chao, T.-K., Sytwu, H.-K., Uchida, S., Sasaki, S., Lin, S.-H. Generation and analysis of the thiazide-sensitive Na(+)-Cl(-) cotransporter (Ncc/Slc12a3) Ser707X knockin mouse as a model of Gitelman syndrome. Hum. Mutat. 31: 1304-1315, 2010.

SLC12A3

Solute carrier family 12 member 3

solute carrier family 12(sodium/chloride transporters), member 3

FLJ96318 antibody Na Cl symporter antibody Na-Cl symporter antibody NaCl electroneutral thiazide sensitive cotransporter antibody NCCT antibody S12A3_HUMAN antibody slc12a3 antibody Solute carrier family 12(sodium/chloride transporters) member 3 antibody Solute carrier family 12 member 3 antibody Thiazide sensitive Na Cl cotransporter antibody Thiazide sensitive sodium chloride cotransporter antibody Thiazide-sensitive sodium-chloride cotransporter antibody TSC antibody

P55017