Boster Immunoleader

antibody

Anti-NKCC1 Antibody

PA2169

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-NKCC1 Antibody

PA2169

Polyclonal

Rabbit IgG polyclonal antibody for Solute carrier family 12 member 2(SLC12A2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P,IHC-F

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Rat; Predicted Species: Human, Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and IHC(F).

Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.

1. Dzhala, V. I., Talos, D. M., Sdrulla, D. A., Brumback, A. C., Mathews, G. C., Benke, T. A., Delpire, E., Jensen, F. E., Staley, K. J. NKCC1 transporter facilitates seizures in the developing brain. Nature Med. 11: 1205-1213, 2005. 2. Nguyen, M., Pace, A. J., Koller, B. H. Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia. J. Exp. Med. 204: 1383-1393, 2007.

SLC12A2

Solute carrier family 12 member 2

solute carrier family 12(sodium/potassium/chloride transporters), member 2

Basolateral Na-K-Cl symporter antibody BSC antibody BSC2 antibody Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody mBSC2 antibody MGC104233 antibody NKCC1 antibody S12A2_HUMAN antibody SLC12A2 antibody sodium-potassium-chloride cotransporter 1 antibody solute carrier family 12(sodium/potassium/chloride transporters) member 2 antibody Solute carrier family 12 member 2 antibody sy-ns antibody

P55011