Anti-Transglutaminase 2 Antibody | Boster Immunoleader PA2153 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Transglutaminase 2 Antibody

catalog :

PA2153

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

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product information

Product Name :

Anti-Transglutaminase 2 Antibody

Catalog Number :

PA2153

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Protein-glutamine gamma-glutamyltransferase 2(TGM2) detection. Tested with WB, IHC-P, ICC in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human, rat

Applications :

WB,IHC-P,ICC

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat

Immunohistochemistry(Paraffin-embedded S ... :

Concentration: 0.5-1μg/ml; Tested Species: Human

Immunocytochemistry :

Concentration: 0.5-1μg/ml; Tested Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Transglutaminase 2(399-418aa ADVVDWIQQDDGSVHKSINR), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

Background :

Tissue transglutaminase, also called TGC, is a 78-kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization(FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.

Reference :

1. Antonyak, M. A., Jansen, J. M., Miller, A. M., Ly, T. K., Endo, M., Cerione, R. A. Two isoforms of tissue transglutaminase mediate opposing cellular fates. Proc. Nat. Acad. Sci. 103: 18609-18614, 2006. 2. Mastroberardino, P. G., Iannicola, C., Nardacci, R., Bernassola, F., de Laurenzi, V., Melino, G., Moreno, S., Pavone, F., Oliverio, S., Fesus, L., Piacentini, M. 'Tissue' transglutaminase ablation reduces neuronal death and prolongs survival in a mouse model of Huntington's disease. Cell Death Differ. 9: 873-880, 2002. 3. Priglinger, S. G., May, C. A., Neubauer, A. S., Alge, C. S., Schoenfeld, C.-L., Kampik, A., Welge-Lussen, U. Tissue transglutaminase as a modifying enzyme of the extracellular matrix in PVR membranes. Invest. Ophthal. Vis. Sci. 44: 355-364, 2003.

Gene Name :

TGM2

Protein Name :

Protein-glutamine gamma-glutamyltransferase 2

Gene Full Name :

transglutaminase 2

Synonyms :

ALPHA SUBUNIT antibody C polypeptide antibody EC 2.3.2.13 antibody G alpha h antibody G protein alpha subunit Gh class antibody G[a]h antibody Gh CLASS G ALPHA h antibody GNAH antibody GNAH G PROTEIN antibody Guanine nucleotide binding protein H polypeptide antibody H POLYPEPTIDE antibody Protein glutamine gamma glutamyltransferase 2 antibody Protein glutamine gamma glutamyltransferase antibody Protein-glutamine gamma-glutamyltransferase 2 antibody TG 2 antibody TG(C) antibody TG2 antibody TGase C antibody TGase H antibody TGase-2 antibody TgaseII antibody TGC antibody TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody TGM 2 antibody TGM2 antibody TGM2_HUMAN antibody Tissue transglutaminase antibody Tissue type transglutaminase antibody Transglutaminase 2 antibody Transglutaminase 2 C polypeptide antibody Transglutaminase C antibody Transglutaminase H antibody Transglutaminase-2 antibody tTG antibody tTGas antibody

Uniprot ID :

P21980

more info or order :

Boster Immunoleader product webpage