Anti-HIF3 Antibody | Boster Immunoleader PA2145 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-HIF3 Antibody

catalog :

PA2145

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-HIF3 Antibody

Catalog Number :

PA2145

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 3-alpha(HIF3A) detection. Tested with WB in Human;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: human

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Human

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3(497-514aa DDDFQLNASEQLPRAYHR), different from the related rat and mouse sequences by five amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element(HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Reference :

1. Hara, S., Hamada, J., Kobayashi, C., Kondo, Y., Imura, N. Expression and characterization of hypoxia-inducible factor (HIF)-3-alpha in human kidney: suppression of HIF-mediated gene expression by HIF-3-alpha. Biochem. Biophys. Res. Commun. 287: 808-813, 2001. 2. Maynard, M. A., Qi, H., Chung, J., Lee, E. H. L., Kondo, Y., Hara, S., Conaway, R. C., Conaway, J. W., Ohh, M. Multiple splice variants of the human HIF-3-alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J. Biol. Chem. 278: 11032-11040, 2003.

Gene Name :

HIF3A

Protein Name :

Hypoxia-inducible factor 3-alpha

Gene Full Name :

hypoxia inducible factor 3, alpha subunit

Synonyms :

Basic-helix-loop-helix-PAS protein MOP7 antibody bHLHe17 antibody Class E basic helix-loop-helix protein 17 antibody HIF 3A antibody HIF 3A4 antibody HIF-3-alpha antibody HIF3 alpha antibody HIF3-alpha antibody HIF3-alpha-1 antibody HIF3A antibody HIF3A_HUMAN antibody Hypoxia Inducible Factor 3 alpha antibody Hypoxia inducible factor 3 alpha subunit antibody Hypoxia inducible factor three alpha antibody Hypoxia-inducible factor 3-alpha antibody Inhibitory PAS domain protein antibody IPAS antibody Member of PAS protein 7 antibody MOP7 antibody PAS domain-containing protein 7 antibody PASD7 antibody

Uniprot ID :

Q9Y2N7

more info or order :

Boster Immunoleader product webpage