Anti-E2F3 Antibody | Boster Immunoleader PA2142 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-E2F3 Antibody

catalog :

PA2142

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-E2F3 Antibody

Catalog Number :

PA2142

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Transcription factor E2F3(E2F3) detection. Tested with WB in Human;Mouse.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3(446-465aa DLFDAYDLEKLPLVEDFMCS), identical to the related mouse sequence.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein(pRB) to regulate the expression of genes involved in the cell cycle.

Reference :

1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009.

Gene Name :

E2F3

Protein Name :

Transcription factor E2F3

Gene Full Name :

E2F transcription factor 3

Synonyms :

DKFZp686C18211 antibody E2F 3 antibody E2F transcription factor 3 antibody E2F-3 antibody E2F3 antibody E2F3_HUMAN antibody KIAA0075 antibody MGC104598 antibody Transcription factor E2F 3 antibody Transcription factor E2F3 antibody

Uniprot ID :

O00716

more info or order :

Boster Immunoleader product webpage