Anti-Collagen II Antibody | Boster Immunoleader PA2141 product information

product summary

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company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Collagen II Antibody

catalog :

PA2141

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat

application :

western blot

publications citing this reagent :

Han Z, Zhang H, Wang Y, Lv C, Xu Z. Effect of the human insulin-like growth factor 1 gene transfection to human umbilical cord blood mesenchymal stem cells. Saudi Med J. 2014;35:435-41 pubmed
Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, et al. Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons. In Vitro Cell Dev Biol Anim. 2014;50:304-12 pubmed publisher
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z, et al. Interleukin-18-induced inflammatory responses in synoviocytes and chondrocytes from osteoarthritic patients. Int J Mol Med. 2012;30:805-10 pubmed publisher

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Collagen II Antibody

Catalog Number :

PA2141

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB in Human;Mouse;Rat.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: human Predicted to work with: mouse, rat

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Reference :

1. Donahue, L. R., Chang, B., Mohan, S., Miyakoshi, N., Wergedal, J. E., Baylink, D. J., Hawes, N. L., Rosen, C. J., Ward-Bailey, P., Zheng, Q. Y., Bronson, R. T., Johnson, K. R., Davisson, M. T. A missense mutation in the mouse Col2a1 gene causes spondyloepiphyseal dysplasia congenita, hearing loss, and retinoschisis. J. Bone Miner. Res. 18: 1612-1621, 2003. Note: Erratum: J. Bone Miner. Res. 22: 2011 only, 2007. 2. Faivre, L., Le Merrer, M., Douvier, S., Laurent, N., Thauvin-Robinet, C., Rousseau, T., Vereecke, I., Sagot, P., Delezoide, A.-L., Coucke, P., Mortier, G. Recurrence of achondrogenesis type II within the same family: evidence for germline mosaicism. Am. J. Med. Genet. 126A: 308-312, 2004.

Gene Name :

COL2A1

Protein Name :

Collagen alpha-1(II) chain

Gene Full Name :

collagen, type II, alpha 1

Synonyms :

Alpha 1 type II collagen antibody Alpha-1 type II collagen antibody AOM antibody Cartilage collagen antibody Chondrocalcin antibody CO2A1_HUMAN antibody COL11A3 antibody COL2A1 antibody COL2A1 protein antibody Collagen alpha 1(II) chain precursor antibody Collagen II alpha 1 polypeptide antibody Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody MGC131516 antibody SEDC antibody

Uniprot ID :

P02458

more info or order :

Boster Immunoleader product webpage