Boster Immunoleader

antibody

Anti-ARID1A Antibody

PA2138

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-ARID1A Antibody

PA2138

Polyclonal

Rabbit IgG polyclonal antibody for AT-rich interactive domain-containing protein 1A(ARID1A) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P,ICC

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human; Predicted Species: Mouse, Rat

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the C-terminus of human ARID1A(2199-2213aa LEDSLAATQFQQSQA), identical to the related mouse and rat sequences.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P) and ICC.

AT-rich interactive domain-containing protein 1A, also known as p270, is a protein that in humans is encoded by the ARID1A gene. This gene encodes a member of the SWI/SNF families, whose members have helicase and ATPase activities and are though to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is mapped to 1p36.11. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation.

1. Dallas, P. B., Pacchione, S., Wilsker, D., Bowrin, V., Kobayashi, R., Moran, E. The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity. Molec. Cell. Biol. 20: 3137-3146, 2000. 2. Krosl, J., Mamo, A., Chagraoui, J., Wilhelm, B. T., Girard, S., Louis, I., Lessard, J., Perreault, C., Sauvageau, G. A mutant allele of the Swi/Snf member BAF250a determines the pool size of fetal liver hemopoietic stem cell populations. Blood 116: 1678-1684, 2010. 3. Nie, Z., Xue, Y., Yang, D., Zhou, S., Deroo, B. J., Archer, T. K., Wang, W. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Molec. Cell. Biol. 20: 8879-8888, 2000.

ARID1A

AT-rich interactive domain-containing protein 1A

AT rich interactive domain 1A(SWI-like)

actin-dependent regulator of chromatin subfamily F member 1 antibody ARI1A_HUMAN antibody ARID domain containing protein 1A antibody ARID domain-containing protein 1A antibody ARID1A antibody AT rich interactive domain 1A(SWI like) antibody AT rich interactive domain 1A antibody AT rich interactive domain containing protein 1A antibody AT-rich interactive domain-containing protein 1A antibody B120 antibody BAF250 antibody BAF250A antibody BM029 antibody brain protein 120 antibody BRG1 associated factor 250 antibody BRG1 associated factor 250a antibody BRG1-associated factor 250 antibody BRG1-associated factor 250a antibody C1ORF4 antibody chromatin remodeling factor p250 antibody chromosome 1 open reading frame 4 antibody hELD antibody hOSA1 antibody matrix-associated antibody Osa homolog 1 antibody OSA1 antibody OSA1 nuclear protein antibody P270 antibody SMARCF1 antibody SWI like protein antibody SWI SNF complex protein p270 antibody SWI-like protein antibody SWI/SNF complex protein p270 antibody SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 antibody SWI/SNF-related antibody

O14497