Boster Immunoleader

antibody

Anti-Syndecan 3 Antibody

PA2133

100 ug/vial

200 USD

polyclonal

rabbit

human, mouse, rat

western blot, immunohistochemistry - paraffin section

Anti-Syndecan 3 Antibody

PA2133

Polyclonal

Rabbit IgG polyclonal antibody for Syndecan-3(SDC3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

200 USD

Rabbit IgG

100 ug/vial

Lyophilized

No cross reactivity with other proteins.

Reacts with: human, rat Predicted to work with: mouse

WB,IHC-P

Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Concentration: 0.5-1μg/ml; Tested Species: Human, Rat; Predicted Species: Mouse

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Syndecan 3(45-60aa AQRWRNENFERPVDLE), identical to the related rat sequence, and different from the related human sequence by one amino acid.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).

Syndecan-3, also called SYND3 is a protein that in humans is encoded by the SDC3 gene. This gene was assigned to human chromosome 1p35.2. This gene can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparin sulfate chains. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, and possibly by transferring signals from the cell surface in a sugar-dependent mechanism. In wildtype mice, SDC3 was expressed in hypothalamic regions that control energy balance. It was proposed that oscillation of hypothalamic SDC3 levels physiologically modulates feeding behavior.

1. Berndt, C., Casaroli-Marano, R. P., Vilaro, S., Reina, M. Cloning and characterization of human syndecan-3. J. Cell. Biol. 82: 246-259, 2001. 2. Bobardt, M. D., Saphire, A. C. S., Hung, H.-C., Yu, X., Van der Schueren, B., Zhang, Z., David, G., Gallay, P. A. Syndecan captures, protects, and transmits HIV to T lymphocytes. Immunity 18: 27-39, 2003. 3. de Witte, L., Bobardt, M., Chatterji, U., Degeest, G., David, G., Geijtenbeek, T. B. H., Gallay, P. Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1.Proc. Nat. Acad. Sci. 104: 19464-19469, 2007. 4. Ha, E., Kim, M.-J., Choi, B.-K., Rho, J.-J., Oh, D.-J., Rho, T.-H., Kim, K.-H., Lee, H. J., Shin, D.-H., Yim, S. V., Baik, H. H., Chung, J.-H., Kim, J. W. Positive association of obesity with single nucleotide polymorphisms of syndecan 3 in the Korean population. J. Clin. Endocr. Metab. 91: 5095-5099, 2006.

SDC3

Syndecan-3

syndecan 3

N-syndecan antibody Sdc3 antibody SDC3_HUMAN antibody SDCN antibody SYND3 antibody syndecan proteoglycan 3 antibody Syndecan-3 antibody

Q64519