Anti-Cdc20 Antibody | Boster Immunoleader PA2124 product information

product summary

request information :

company name :

Boster Immunoleader

product type :

antibody

product name :

Anti-Cdc20 Antibody

catalog :

PA2124

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

reactivity :

mouse, rat

application :

western blot

more info or order :

Boster Immunoleader product webpage

product information

Product Name :

Anti-Cdc20 Antibody

Catalog Number :

PA2124

Clonality :

Polyclonal

Description short :

Rabbit IgG polyclonal antibody for Cell division cycle protein 20 homolog(CDC20) detection. Tested with WB in Rat;Mouse.

Price :

200 USD

Ig type :

Rabbit IgG

Size :

100 ug/vial

Form :

Lyophilized

Specificity :

No cross reactivity with other proteins.

Reactivity :

Reacts with: rat Predicted to work with: mouse

Applications :

Western blot :

Concentration: 0.1-0.5μg/ml; Tested Species: Rat; Predicted Species: Mouse

Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cdc20(20-36aa IPNAPVARWQRKAKEAT), different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids.

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage :

At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.

Relevant detection systems :

Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.

Background :

The cell-division cycle protein 20, also known as p55CDC, is an essential regulator of cell division that is encoded by the CDC20 gene in humans. The chromosomal assignment of human CDC20 is 1p34.2. CDC20 is a component of the mammalian cell cycle mechanism. CDC20 appears to act as a regulatory protein interacting with many other proteins at multiple points in the cell cycle. This gene's most important function is to activate the anaphase promoting complex(APC), a large 11-13 subunit complex that initiates chromatid separation and entrance into anaphase.

Reference :

1. Herzog, F., Primorac, I., Dube, P., Lenart, P., Sander, B., Mechtler, K., Stark, H., Peters, J.-M. Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science 323: 1477-1481, 2009. 2. Morris, M. C., Kaiser, P., Rudyak, S., Baskerville, C., Watson, M. H., Reed, S. I. Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 423: 1009-1013, 2003. 3. Reddy, S. K., Rape, M., Margansky, W. A., Kirschner, M. W. Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation. Nature 446: 921-925, 2007. 4. Yang, Y., Kim, A. H., Yamada, T., Wu, B., Bilimoria, P. M., Ikeuchi, Y., de la Iglesia, N., Shen, J., Bonni, A. A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation. Science 326: 575-578, 2009.

Gene Name :

CDC20

Protein Name :

Cell division cycle protein 20 homolog

Gene Full Name :

cell division cycle 20

Synonyms :

bA276H19.3 antibody Cdc 20 antibody CDC20 antibody CDC20 cell division cycle 20 homolog antibody CDC20_HUMAN antibody CDC20A antibody Cell division cycle 20 antibody Cell division cycle 20 homolog(S. cerevisiae) antibody Cell division cycle 20 homolog antibody Cell division cycle protein 20 homolog antibody fizzy antibody MGC102824 antibody p55CDC antibody

Uniprot ID :

Q9JJ66

more info or order :

Boster Immunoleader product webpage